当归红芪水煎剂对糖尿病大鼠肾脏的保护作用

Protective effects of the Danggui Hongqi decoction onkidneys of diabetic rats

张凌伟¹ 锁海虹³  万生芳^1* 王秋兰¹ 于兴旺²  李荣科¹  郭钰龙¹  

（1.甘肃中医学院，甘肃兰州 730000；2.兰州市康复中心医院，甘肃兰州 730050；

 3.河北以岭医院，河北石家庄，050091）

中图分类号：R259    文献标识码：A    文章编号：1674-7860(2015)27-0026-02    证型：DBA

【摘  要】目的：探讨当归红芪水煎剂对糖尿病大鼠肾脏的保护作用。方法：将72只Wistar大鼠随机分为正常对照组12只，其余60只大鼠，给予一次性大剂量腹腔注射链脲佐菌素（STZ）复制糖尿病模型。造模成功后将糖尿病大鼠随机分为厄贝沙坦组（给予17.5mg•kg^-1•d^-1灌胃），当归红芪低剂量组(给予1.8g•kg^-1•d^-1灌胃），当归红芪中剂量组(给予3.6g•kg^-1•d^-1灌胃），当归红芪高剂量组（给予7.2g•kg^-1•d^-1灌胃），模型组（给予等量生理盐水灌胃），每组12只。4周后测空腹血糖（FBG）；用代谢笼留大鼠24h尿，考马斯亮蓝G-250法测定24h尿蛋白含量（24 h Upro）；解剖大鼠，取双肾，称重，计算肾脏指数；采用HE染色观察各组大鼠肾组织的病理变化。结果：与正常对照组比较模型组大鼠FBG、24 hUpro、肾重指数均明显升高（P＜0.01）。与模型组比较，当归红芪中、高剂量组空腹血糖有不同程度的降低（P＜0.05）；西药组及三个当归红芪组24 h Upro均有所下降（P＜0.05）；西药组及当归红芪中、高剂量组肾重指数明显下降（P＜0.05）；西药组及当归红芪中、高剂量组肾脏病理变化较轻（P＜0.05）。结论：当归红芪水煎剂可以降低DM大鼠的血糖、减少蛋白尿、减轻肾脏病理损害，从而可以延缓糖尿病肾病的进展。

【关键词】当归；红芪；糖尿病；肾脏

【Abstract】Objective:To investigate effeccts of the DangguiHongqi decoction on kidney of experimental diabetic rats. Methods: 72 Wistarrats were randomly divided into control group 12, the remaining 60 rats wereadministered bolus intraperitoneal injection of streptozotocin (STZ) diabeticcopy. Later, successful modeling of diabetic rats were randomly divided intoirbesartan group (given 17.5mg•kg^-1•d^-1 orally), DangguiHongqi low dose group (given1.8 g•kg^-1•d^-1 orally), DangguiHongqi dose group (given 3.6 g•kg^-1•d^-1 orally), DangguiHongqi high dose group (given 7.2 g•kg^-1•d^-1 orally),model group (given normal saline orally), each 12. Four weeks after fastingblood glucose (FBG) was done, with 24h urine of rats, and Determinating 24hurinary protein (24h Upro); dissect rats for kidneys, weighing, calculatingkidney index; HE staining for renal tissue pathological changes. Results: Comparedwith normal control group of rats FBG model group, 24hUpro, kidney weight indexwere significantly increased (P＜0.01). Compared with the model group, danggui hongqimiddle and high dose groups have different levels of fasting blood glucoselowering(P＜0.05). Western medicine group and three Danggui Hongqi24h Upro group had decreased(P＜0.05); Western medicine group and Danggui Hongqi middledose group and high dose group significantly decreased kidney weight index(P＜0.05);Western medicine group and Danggui Hongqi middle dose group and high dose groupmild were found renal pathological changes (P＜0.05). Conclusion: Danggui Hongqi decoction can reducethe blood sugar, reduce proteinuria, in DM rats can relieve kidney pathologicaldamage, and can delay the progress of diabetic nephropathy.

【Keywords】Danggui; Hongqi; Diabetes; Kidney

doi:10.3969/j.issn.1674-7860.2015.27.011

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